The microbiome and the effectiveness of supplements: why a healthy gut is key to absorption
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Introduction: Why don't supplements work?
Are you taking a handful of supplements every day, and still not seeing results? The problem may not be in the quality
supplements, but in the state of your gut microbiome - the community of trillions of microorganisms in your digestive tract
digestive tract.
Your gut is home to 10-100 trillion bacteria that are not passive passengers. They actively participate in digestion,
vitamin production, immune regulation and - crucially - in the absorption of nutrients and
supplements.
New research shows something fascinating: the gut microbiota may be responsible for the fact that the same supplement works
perfectly in one person and not at all in another. It's not a matter of fate - it's a matter of the health of your microbiome.
How does the microbiome affect the absorption of supplements?
1. Biotransformation - change of chemical structure
Gut bacteria produce enzymes that humans do not have. These enzymes
can:
Release active ingredients from their inactive forms
Modify the chemical structure of substances,
increasing or decreasing their absorption
Degrade substances before they are absorbed
Studies have shown that the microbiome can modify the structure of as many as 271 different drugs
oral through processes such as oxidation, reduction or hydroxylation.
Example: Resveratrol (a popular antioxidant) is converted by bacteria
intestinal bacteria into metabolites with different biological activities. At the same time, resveratrol changes the composition of the
microbiota, increasing the abundance of beneficial bacteria like Lactobacillus and Bifidobacterium.
2. Effects on transporters - absorption "gates"
Microbiota affects transporter proteins in intestinal cells, which are responsible for absorption of substances.
Animal studies have shown that:
The bacterium Bacteroides thetaiotaomicron reduced the expression of a protein that pumps substances out of cells czterokrotnie
Lactobacillus murinus increased the bioavailability of certain substances by decreasing the expression of
transporters
3. Production of short-chain fatty acids (SCFA)
This is the most important mechanism! SCFAs-acetate, propionate and butyrate-are formed from the fermentation of fiber by intestinal bacteria
intestinal bacteria.
What do SCFAs do?
Acidify the intestinal environment - lowered pH increases solubility and absorption of minerals
(calcium, magnesium, iron, zinc)
They are a source of energy for intestinal cells - butyrate supplies 70% of the energy needs of the
of colonocytes
Strengthen the intestinal barrier - increase the expression of tight junction proteins
Reduce inflammation - have anti-inflammatory effects
It is estimated that the fermentation of 50-60g of carbohydrates per day generates about500-600 mmol of SCFA , which
represents about10% of our daily energy needs .
Leaky gut syndrome: a major enemy of absorption
What is a "leaky gut"?
Under normal conditions, the intestinal wall acts like a sieve - letting beneficial nutrients through,
retaining harmful substances (toxins, bacteria, undigested particles).
The intestinal wall is a sieve
When the tight connections between epithelial cells weaken, microscopic "holes" form, through which
unwanted substances enter the bloodstream, triggering an inflammatory response.
Causes of leaky gut:
Gut dysbiosis (unbalanced microbiome)
Pro-inflammatory diet (sugar, trans fats, processed foods)
Chronic inflammation reduces the expression of digestive enzymes
The surface area of absorption decreases
Pathogenic bacteria "steal" vitamins and minerals
Concrete examples: supplements and microbiome
B vitamins
What the research says: Gut bacteria produce 40-65% of the eight B vitamins
B. Species such as Bacteroides, Bifidobacterium and Lactobacillus synthesize these vitamins from scratch.
Key note: Vitamins must be produced "upstream" of where they are absorbed. Vitamin
B12 is absorbed in the ileum, so B12-producing colon bacteria are unlikely to help
increase its bioavailability to the host.
Iron
Iron is a special case - its supplementation can have both positive and negative effects
skutki:
Positive: Correcting deficiencies improves immune function
Negative: Excess iron promotes the growth of pathogens (e.g., E. coli)
Studies showed that a high dose of iron (6.4 mg/day) significantly reduced the abundance of beneficial
Bifidobacterium compared to a lower dose (1.2 mg/day).
Lesson: Iron supplementation should be carefully dosed, especially in people with
intestinal problems.
Zinc
Zinc is essential for maintaining the integrity of the intestinal barrier.
Badania pokazują:
Zinc deficiency alters the structure of the microbiota, leading to reduced diversity and increased
inflammation
A randomized trial in patients with Crohn's disease showed that supplementation with Zinc
zinc helped "seal" the leaky gut
Carnosine zinc in the study showed impressive improvements in intestinal integrity as early as after 4
weeks
Recommended dosage: 15-30 mg daily (preferably in chelate or carnosine form).
Polyphenols (resveratrol, quercetin, curcumin)
Polyphenols have low bioavailability in unchanged form, but the microbiota converts them into more
bioactive metabolites.
Studies show that phenolic phytochemicals with low bioavailability can reduce levels of
harmful compounds mainly by remodeling the gut microbiota.
How to repair the microbiome and improve absorption?
1. Prebiotics: food for good bacteria
Prebiotics are indigestible dietary fibers that stimulate the growth of beneficial bacteria.
Najlepsze źródła:
Inulin: artichokes, garlic, leeks, onions (5-10g
per day)
FOS : bananas, garlic, asparagus (2-10g per day)
Resistant starch: fried potatoes, green bananas,
beans (15-30g per day)
A 2018 study found that fiber intake inhibited the
growth of harmful bacteria and reduced levels of bacterial toxins in patients
with type 2 diabetes.
2. Probiotics: live beneficial bacteria
Lactobacillus rhamnosus GG:
Strengthens the protection of the intestinal mucosa
Redukuje zapalenie
Dosage: minimum of 10 billion CFU
Bifidobacterium longum:
Protects against pathogens
Produces acetanion and lactate
Promotes the intestinal barrier
Saccharomyces boulardii:
Probiotic yeast
Prevents excessive growth of Candida
Helps with diarrhea
Zalecenia:
Multi-strain formulas (10-50 billion CFU)
Take on an empty stomach or with a light meal
Combine with prebiotics (synbiotics)
3. Supplements that repair the intestinal barrier
L-Glutamina:
Main source of energy for intestinal cells
Repairs tight junctions
Dosage: 5-15g daily
Cynk-karnosyna:
Promotes repair of damaged epithelial cells
Dosing: 75mg 2x daily
Maślan (butyrate):
Promotes colonocyte health
Acts as an anti-inflammatory
Dawkowanie: 150-300mg dziennie
Omega-3 (EPA and DHA):
Reduces inflammation in the mucosa
Promotes barrier integrity
Dosage: 2-3g daily
Kwercetyna:
Reduces intestinal permeability
Protects against oxidative stress
Dawkowanie: 500-1000mg dziennie
4. Diet to support a healthy microbiome
What to eat:
Fermented foods: Sauerkraut, kimchi, kefir, natural yogurt, kombucha →
They provide
live probiotics and postbiotics
Permanent stress changes the composition of the microbiota and weakens the intestinal barrier. → Meditation, yoga, deep
breathing (10-20
min per day)
Regular sleep
Microbiota has its own diurnal rhythm. Sleep disorders lead to dysbiosis. → 7-9 hours of sleep, fixed
schedule,
limiting blue light in the evening
Regular physical activity
Exercise increases microbiota diversity and SCFA production. → 150 minutes of moderate activity
per week
Careful with antibiotics
Antibiotics destroy the microbiome - effects can last for months. → If you must take: add
probiotics (2-3h after antibiotics), increase fiber.
of 2023 in Nature Communications showed that fiber supplementation protects against
against antibiotic-induced dysbiosis
Most common mistakes in supplementation
Error #1: Supplements without intestinal repair
Problem: Damaged barrier = poor absorption Solution: First fix
gut, then optimize supplementation
Mistake #2: Too high doses of probiotics at the beginning
Problem: People with SIBO may not tolerate high
dawki Rozwiązanie: Start with 5-10
billion CFUs and gradually increase
Mistake #3: Ignoring prebiotics
Problem: Probiotics without prebiotics may not colonize the gut Rozwiązanie: Always combine probiotics with prebiotics
Error #4: Lack of patience
Problem: Repairing the microbiome takes time Solution: Minimum 3-6
months
consistent action
Mistake #5: Forgetting about diet
Problem: Supplements are no substitute for a bad diet Solution: 80% success is diet and
lifestyle, 20% is supplementation
Summary: The key to effective supplementation
A healthy gut microbiome and a tight gut barrier are the foundation of effective supplementation . Here is
key findings:
The microbiome directly affects the bioavailability of supplements through biotransformation, modulation of
transporters, SCFA production, and regulation of inflammation
The syndrome of
leaky gut dramatically reduces the absorption of of vitamins, minerals and other nutrients
Repairing the
intestines should be a priority- Repair the barrier first, then optimize supplementation
Kluczowe
supplements for gut health:
Multi-strain microbiotics (25-50 billion CFU)
Prebiotics (5-10g daily)
L-glutamine (5-15g daily)
Carnosine zinc (150mg per day)
Butyrate (150-300mg per day)
Omega-3 (2-3g per day)
Diet
is the foundation - Minimum 30g of fiber per day, fermented foods, polyphenols, avoiding sugar
Patience
is the key- Microbiome repair requires 3-6 months of consistent action
Remember: There is no magic pill. A healthy gut is the result of a holistic approach combining
proper diet, supplementation, stress management and a healthy lifestyle. If you invest in the health of your gut
gut, they will invest in your overall health - better energy, stronger immunity and optimal effectiveness of
supplementation.
Bibliography and sources:
Microbiome and bioavailability studies:
Barone, M., Turroni, S., Rampelli, S., et al. (2022). "Gut microbiome-micronutrient interaction: the key
to controlling the bioavailability of minerals and vitamins?" BioFactors 48(2):307-314. DOI:
10.1002/biof.1835
Das, P., Babaei, P., Nielsen, J. (2019). "Metagenomic analysis of microbe-mediated vitamin metabolism in
the human gut microbiome." BMC Genomics 20:208 DOI: 10.1186/s12864-019-5591-7
Zhang, X., Han, Y., Huang, W., et al. (2021). "The influence of the gut microbiota on the
bioavailability of oral drugs." Acta Pharmaceutica Sinica B 11(7):1789-1812. DOI:
10.1016/ j.apsb.2020.09.013
Li, L., Somerset, S. (2018). "Bioavailability Based on the Gut Microbiota: a New Perspective."
Microbiology and Molecular Biology Reviews 82(4):e00016-18. DOI: 10.1128/MMBR.00016-18
Spanogiannopoulos, P., Bess, E.N ., Carmody, R.N ., Turnbaugh, P.J. (2016). "The microbial pharmacists
within us: a metagenomic view of xenobiotic metabolism." Nature Reviews Microbiology 14:273-287. DOI:
10.1038/nrmicro.2016.17
Research on SCFA:
Koh, A., De Vadder, F., Kovatcheva-Datchary, P., Bäckhed, F. (2016). "From dietary fiber to host
physiology: Short-chain fatty acids as key bacterial metabolites." Cell 165(6):1332-1345. DOI:
10.1016/j.cell.2016.05.041
den Besten, G., van Eunen, K., Groen, A.K., et al. (2013). "The role of short-chain fatty acids in the
interplay between diet, gut microbiota, and host energy metabolism." Journal of Lipid Research
54(9):2325-2340. DOI: 10.1194/ jlr.R036012
Silva,Y.P ., Bernardi, A., Frozza, R.L . (2020). "The Role of Short-Chain Fatty Acids From Gut Microbiota
in Gut-Brain Communication." Frontiers in Endocrinology 11:25. DOI: 10.3389/fendo.2020.00025
Oliphant, K., Allen-Vercoe, E. (2024). "Short-Chain Fatty Acids and Human Health: From Metabolic
Pathways to Current Therapeutic Implications." Life 14(5):559. DOI: 10.3390/life14050559
Entestinal barrier research:
Camilleri, M. (2019). "Leaky gut: mechanisms, measurement and clinical implications in humans" Gut
68(8):1516-1526. DOI: 10.1136/gutjnl-2019-318427
Bischoff, S.C., Barbara, G., Buurman, W., et al. (2014). "Intestinal permeability - a new target for
disease prevention and therapy." BMC Gastroenterology 14:189. DOI: 10.1186/s12876-014-0189-7
Martínez Leo, E.E ., Segura Campos, M.R. (2023). "Leaky Gut and the Ingredients That Help Treat It: A
Review." Molecules 28(2):619. DOI: 10.3390/molecules28020619
Research on probiotics and prebiotics:
Hill, C., Guarner, F., Reid, G., et al. (2014). "Expert consensus document: the International Scientific
Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the
term probiotic." Nature Reviews Gastroenterology & Hepatology 11:506-514. DOI:
10.1038/nrgastro.2014.66
Gibson, G.R., Hutkins, R., Sanders, M.E., et al. (2017). "Expert consensus document: the International
Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and
scope of prebiotics." Nature Reviews Gastroenterology & Hepatology 14:491-502. DOI:
10.1038/nrgastro.2017.75
Korry, B.J ., Cabral, D.J., Belenky, P. (2023). "Fiber supplementation protects from antibiotic-induced
gut microbiome dysbiosis by modulating gut redox potential." Nature Communications 14:5161. DOI:
10.1038/s41467-023-40553-x
Research on specific supplements:
Sturniolo, G.C., Di Leo, V., Ferronato, A., et al. (2001). "Zinc supplementation tightens 'leaky gut' in
Crohn's disease." Inflammatory Bowel Diseases 7(2):94-98. DOI: 10.1097/00054725-200105000-00003
Benjamin, J., Makharia, G., Ahuja, V., et al. (2012). "Glutamine and whey protein improve intestinal
permeability and morphology in patients with Crohn's disease: a randomized controlled trial." Digestive
Diseases and Sciences 57(4):1000-1012. DOI: 10.1007/s10620-011-1947-9
Zhao, L., Zhang, F., Ding, X., et al. (2018). "Gut bacteria selectively promoted by dietary fibers
alleviate type 2 diabetes." Science 359(6380):1151-1156. DOI: 10.1126/ science.aao5774
Kumar Singh, A., Cabral, C., Kumar, R., et al. (2019). "Beneficial Effects of Dietary Polyphenols on Gut
Microbiota and Strategies to Improve Delivery Efficiency." Nutrients 11(9):2216. DOI: 10.3390/nu11092216
Rinninella, E., Raoul, P., Cintoni, M., et al. (2019). "What is the Healthy Gut Microbiota Composition?
A Changing Ecosystem across Age, Environment, Diet, and Diseases." Microorganisms 7(1):14. DOI:
10.3390/microorganisms7010014
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